|
KMID : 0861020190340040001
|
|
Korea Journal of Herbology
2019 Volume.34 No. 4 p.1 ~ p.8
|
|
|
DNA barcoding analysis of Rosase Multiflorae Fructus and its adulterants
|
|
Do Eui-Jeong
Shin Sang-Mun
Lee Guem-San
|
|
|
Abstract
|
|
|
Objectives : Rosae Multiflorae Fructus is a traditional medicine derived from the fruit of Rosa multiflora Thunb. a member of the Rosaceae family. Even though it has a single origin, the possibility of adulterants has always existed. In fact, we had discovered suspicious commercial samples of Rosae Multiflorae Fructus, imported from China.
Methods : To define the taxonomic origin of Rosae Multiflorae Fructus and its adulterants, DNA barcode analysis of the internal transcribed spacer, trnL-F intergenic spacer, and psbA-trnH sequences was carried out. These DNA barcode sequences from the correct origin of Rosae Multiflorae Fructus were analyzed and compared with those of other samples from genus Rosa used as medicinal herbs.
Results : The analyses of the three DNA barcode sequences efficiently distinguished Rosae Multiflorae Fructus from six other species in genus Rosa and also separated each species used in this study. According to the DNA barcoding results, none of the suspicious commercial samples were Rosae Multiflorae Fructus. RMF09 was identified as Rosa acicularis, whereas RMF10 and RMF11 were identified as Rosa davurica and Rosa rugosa, respectively. These results corroborated the existence of adulterants of Rosae Multiflorae Fructus.
Conclusions : Our research provides useful information that could be used as a criterion for distinguishing between Rosae Multiflorae Fructus and its adulterants. These results will help in the prevention of adulteration and also suggest effective methods for verifying the origin of commercial herbal medicines derived from genus Rosa.
|
|
|
KEYWORD
|
|
|
authentication, DNA barcoding, Rosase Multiflorae Fructus, Rosaceae, ITS
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|